# Chemical Defenses-5: Park and Dam 2021 # Cell-growth gene expression reveals a direct fitness cost of grazer-inoflagelltoxin production in red tide dinoflagellate prey # PI: Hans G. Dam # Version date: 2021-06-16 ========================= Name Day Hour Cell_Number Growth_Rate Toxin_Content utox Rtox GTX3 GTX4 NEO STX C2 RGE_stxA4 RGE_stxG RGE_cyc g g_modified Cm Cm_modified I I_modified ------------------------- Control 0 0 250 nd 24 nd nd 6 5 7 0 6 1 1 1 nd nd nd nd nd nd Control 0 0 250 nd 45 nd nd 8 13 8 0 17 1 1 1 nd nd nd nd nd nd Control 0 0 250 nd nd nd nd nd nd nd nd nd 1 1 1 nd nd nd nd nd nd Control 0 0 250 nd 35 nd nd nd nd nd nd nd 1 1 1 nd nd nd nd nd nd Control 0.166 4 240 -0.25 41 0.71 27 7 9 14 0 11 0.36 0.83 0.16 nd nd nd nd nd nd Control 0.166 4 245 -0.12 46 1.56 62 8 14 8 0 17 0.21 0.83 0.24 nd nd nd nd nd nd Control 0.166 4 250 0 nd nd nd nd nd nd nd nd 0.11 0.13 0.44 nd nd nd nd nd nd Control 0.166 4 255 0.12 nd nd nd nd nd nd nd nd 9.73 0.23 0.61 nd nd nd nd nd nd Control 0.333 8 235 -0.19 44 0.51 20 8 12 7 0 16 0.59 0.59 0.53 nd nd nd nd nd nd Control 0.333 8 240 -0.12 46 0.71 28 10 10 12 0 14 0.62 0.62 0.61 nd nd nd nd nd nd Control 0.333 8 240 -0.12 nd nd nd nd nd nd nd nd 0.72 0.72 0.78 nd nd nd nd nd nd Control 0.333 8 260 0.12 nd nd nd nd nd nd nd nd 0.64 0.64 1.37 nd nd nd nd nd nd Control 1 24 230 -0.08 47 0.22 9 14 10 10 0 14 0.23 0.52 0.86 nd nd nd nd nd nd Control 1 24 255 0.02 51 0.4 17 9 13 13 0 16 0.11 0.43 0.74 nd nd nd nd nd nd Control 1 24 285 0.13 nd nd nd nd nd nd nd nd 0.07 0.08 1.11 nd nd nd nd nd nd Control 1 24 290 0.15 nd nd nd nd nd nd nd nd 6.37 0.15 1.11 nd nd nd nd nd nd Control 2 48 265 0.03 43 0.14 5 11 9 11 0 12 0.76 0.76 0.76 nd nd nd nd nd nd Control 2 48 270 0.04 53 0.25 11 8 14 14 0 17 0.83 0.83 0.83 nd nd nd nd nd nd Control 2 48 295 0.08 nd nd nd nd nd nd nd nd 1.02 1.02 1.02 nd nd nd nd nd nd Control 2 48 360 0.18 nd nd nd nd nd nd nd nd 1.19 1.19 1.19 nd nd nd nd nd nd Control 3 72 300 0.06 43 0.13 5 11 10 9 0 12 0.07 0.16 0.44 nd nd nd nd nd nd Control 3 72 315 0.08 70 0.31 16 11 23 12 0 25 0.04 0.15 0.57 nd nd nd nd nd nd Control 3 72 325 0.09 nd nd nd nd nd nd nd nd 0.05 0.06 0.69 nd nd nd nd nd nd Control 3 72 330 0.09 nd nd nd nd nd nd nd nd 4.54 0.11 0.99 nd nd nd nd nd nd Control 4 96 320 0.06 26 -0.01 0 6 6 7 0 6 0.09 0.31 0.52 nd nd nd nd nd nd Control 4 96 405 0.12 94 0.37 23 14 36 15 0 29 0.05 0.3 0.55 nd nd nd nd nd nd Control 4 96 415 0.13 nd nd nd nd nd nd nd nd 0.24 0.48 0.73 nd nd nd nd nd nd Control 4 96 435 0.14 nd nd nd nd nd nd nd nd 4.49 0.45 0.96 nd nd nd nd nd nd Treatment 0 0 250 nd nd nd nd nd nd nd nd nd nd nd nd nd nd nd nd nd nd Treatment 0 0 250 nd nd nd nd nd nd nd nd nd nd nd nd nd nd nd nd nd nd Treatment 0 0 250 nd nd nd nd nd nd nd nd nd nd nd nd nd nd nd nd nd nd Treatment 0 0 250 nd nd nd nd nd nd nd nd nd nd nd nd nd nd nd nd nd nd Treatment 0.166 4 225 -0.63 57 2.4 108 9 11 20 0 16 7.94 0.61 0.24 0.39 0.35 237 238 2306 2107 Treatment 0.166 4 235 -0.37 62 3.1 145 10 12 23 0 17 8.4 0.93 0.27 0.25 0.22 242 243 1521 1315 Treatment 0.166 4 249 -0.02 nd nd nd nd nd nd nd nd 9.42 0.63 0.34 0.02 -0.01 249 250 151 -65 Treatment 0.166 4 249 -0.02 nd nd nd nd nd nd nd nd 12.64 0.55 0.35 0.14 0.11 249 250 895 681 Treatment 0.333 8 220 -0.38 58 1.19 54 13 11 19 0 14 13.92 3.92 0.51 0.2 0.13 235 237 1162 764 Treatment 0.333 8 225 -0.32 73 1.9 97 16 14 25 0 19 15.38 3.33 0.53 0.19 0.12 237 240 1150 747 Treatment 0.333 8 225 -0.32 nd nd nd nd nd nd nd nd 12.87 1.16 0.58 0.19 0.12 237 240 1150 747 Treatment 0.333 8 235 -0.19 nd nd nd nd nd nd nd nd 9.09 1.93 0.64 0.3 0.23 242 245 1840 1436 Treatment 1 24 210 -0.17 138 1.21 88 55 20 33 13 16 53.28 8.46 0.49 0.09 0.03 229 237 522 164 Treatment 1 24 215 -0.15 144 1.28 96 59 21 35 13 16 62.61 6.51 0.55 0.17 0.11 232 239 990 643 Treatment 1 24 230 -0.08 nd nd nd nd nd nd nd nd 51.64 7.14 0.71 0.21 0.15 240 247 1286 936 Treatment 1 24 235 -0.06 nd nd nd nd nd nd nd nd 55.84 5.95 0.66 0.21 0.15 242 250 1275 920 Treatment 2 48 200 -0.11 300 0.97 115 158 42 59 26 15 4 9.87 0.54 0.14 0.09 224 236 788 521 Treatment 2 48 215 -0.08 311 1.02 126 165 44 60 26 16 17.9 7.17 0.57 0.11 0.06 232 244 661 376 Treatment 2 48 220 -0.06 nd nd nd nd nd nd nd nd 1.25 6.92 0.59 0.15 0.09 235 247 861 583 Treatment 2 48 235 -0.03 nd nd nd nd nd nd nd nd 10.28 5.25 0.8 0.21 0.16 242 255 1292 1028 Treatment 3 72 205 -0.07 436 0.78 119 240 64 77 35 19 0.93 1.24 0.39 0.13 0.08 227 243 720 490 Treatment 3 72 215 -0.05 456 0.81 128 260 65 77 37 17 0.24 1.85 0.39 0.13 0.08 232 248 739 504 Treatment 3 72 215 -0.05 nd nd nd nd nd nd nd nd 5.77 1.18 0.5 0.14 0.09 232 248 799 569 Treatment 3 72 220 -0.04 nd nd nd nd nd nd nd nd 0.73 0.72 0.47 0.14 0.09 235 251 793 559 Treatment 4 96 200 -0.06 585 0.65 121 315 93 97 48 31 0 2.7 0.4 0.12 0.06 224 253 658 351 Treatment 4 96 200 -0.06 763 0.72 161 416 116 147 55 29 2.1 1.82 0.4 0.18 0.11 224 253 988 724 Treatment 4 96 220 -0.03 nd nd nd nd nd nd nd nd 0 0.46 0.43 0.16 0.1 235 266 931 642 Treatment 4 96 220 -0.03 nd nd nd nd nd nd nd nd 2.89 1.41 0.43 0.17 0.11 235 266 1000 720